Ask AI: write a protocol using human serum to detect 3R and 4R via immune-PCR in steps

1. Obtain a sample of human serum. 2. Separate the serum into two aliquots, one for each allele (3R and 4R). 3. Using a PCR reaction, amplify the targeted DNA region of each allele. 4. Utilize an immobilized monoclonal antibody specific to 3R and 4R respectively to capture the amplified DNA to separate solid support. 5. Follow with a second PCR using a labeled primer containing a fluorescent reporter molecule, such as a fluorescent dye or a biotin-streptavidin. 6. Analyze the PCR product on an appropriate system for detection of the fluorescent signals for 3R and 4R. 7. Interpret results and record.