Ask AI: write a protocol to detect Tau using IPCR in steps focusing on the individual steps in IPCR

1. Design primers to target the sequence of interest: Use a computer program to generate primers that can specifically target the Tau gene. The primers should bear a low probability of forming secondary structures and should have a high GC content. 2. Prepare PCR Reaction Mix: Combine the appropriate amounts of the following components into a single reaction mix: primer mix, DNA template to be amplified, Taq polymerase, dNTPs, and buffer. 3. Set up and Run the IPCR: Load the PCR reaction mix into a thermal cycler and run the appropriate program. Generally, the PCR should include three temperature steps: denaturation, annealing, and extension. 4. Separate and Purify the Amplicons: After the PCR runs, the amplicons must be separated from the reaction mix using gel electrophoresis. Purify the bands using a gel purification kit and elute the DNA from the column. 5. Identify the Amplified Product: Use PCR or conventional Sanger sequencing to identify the amplicon. If a positive band is detected for the Tau gene then it indicates that the gene is present in the sample.