Ask AI: write a protocol for a immuno-PCR assay to measure the trace amounts of 3R and 4R Tau isoforms in steps

1. Obtain appropriate amounts of brain tissue from a 3R and 4R Tau mouse model. 2. Isolate and purify the protein of interest using immunoprecipitation (IP) and SDS-PAGE. 3. Amplify the purified protein samples using a polymerase chain reaction (PCR) approach. 4. Run the samples through a gel electrophoresis analysis to separate and detect the 3R and 4R Tau isoforms. 5. Use a quantitative PCR (qPCR) assay or a real-time PCR (RT-PCR) assay to measure the relative abundance of the 3R and 4R Tau isoforms. 6. Determine the concentration of the 3R and 4R Tau isoforms by comparing the quantitative values to a standard curve. 7. Interpret the results and generate a final report.