Ask AI: What techniques could you use to check for expression of key differentiation markers? What complementary information would they provide?

1. Immunofluorescence Microscopy: This technique can be used to directly visualize differential expression of proteins within different cell types. It can provide an in-depth analysis of protein localization, abundance, and expression levels within cells. 2. Flow Cytometry: This technique can be used to measure the expression level of a protein in a population of cells. It is useful for quantifying the expression of key differentiation markers in different cell types. 3. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR): This technique can be used to detect the expression level of a specific mRNA sequence. It can provide complementary information to immunofluorescence and flow cytometry, as it allows for the detection of both transcriptional and post-transcriptional regulation of gene expression.