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Question: propose an experiment to functionally test if a compound you found can influence the activity of SASP
The proposed experiment would involve using cells that have been genetically engineered to produce the compound that has been identified, and cells that are not genetically engineered as a control. These cells would then be grown in culture until they reach confluence. Then, a SASP factor of interest could be added to each of the cells, and the effects of the compound on the activity of this SASP factor could be monitored. A range of concentrations of the compound could be tested, and the cell culture medium from the treated cells could be tested for the presence of the SASP factor to assess its activity. If the activity of the SASP factor is increased in the presence of the compound, this would indicate that the compound does influence the activity of the SASP factor.
Jan. 14, 2023, 8:47 a.m.
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