Ask AI: prepare a protocol for immunohistochemistry using human CSF from donors in steps. staining was done using 6e10 antibody to identify amyloid plaques

1. Obtain human CSF samples from donor and store at 4°C prior to use. 2. Prepare a 1:100 dilution of 6e10 antibody in 1X PBS. 3. Label a slide for each sample and place a few drops of diluted antibody onto the slides. 4. Place each slide onto a heated slide warmer and allow to sit for 30 minutes, to allow for optimal antibody binding. 5. Wash each slide with 1X PBS and remove any excess antibody. 6. Place the slides in a 10% buffered formalin solution and incubate in the refrigerator for 1 hour. 7. After 1 hour, remove the slides from the formalin solution and rinse with 1X PBS. 8. Prepare a 1:1000 dilution of avidin-biotin-peroxidase complex in 1X PBS. 9. Place a few drops of the avidin-biotin-peroxidase complex onto each slide and incubate in the refrigerator for 30 minutes. 10. Wash each slide with 1X PBS and remove any excess avidin-biotin-peroxidase complex. 11. Place the slides in a freshly prepared diaminobenzidine (DAB) solution and incubate in the refrigerator for 10 minutes. 12. Remove the slides from the DAB solution, rinse with 1X PBS, and then counter stain with hematoxylin. 13. Allow the slides to dry overnight and then cover slide with a coverslip. 14. Visualize the slides with an inverted microscope to detect any amyloid plaques.