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Question: prepare 25% ethylenediamine from 99% ethylenediamine
To prepare a 25% ethylenediamine solution from a 99% ethylenediamine stock solution, you will need to dilute the stock solution appropriately. Here's a step-by-step guide: 1. Determine the desired volume of the 25% ethylenediamine solution you want to prepare. For this example, let's assume you want to prepare 100 mL of the solution. 2. Calculate the amount of 99% ethylenediamine needed: - For a 25% solution in 100 mL, 25 grams of ethylenediamine is required. To find out how much of the 99% solution you need, you can use the following formula: (Amount to prepare) = (Desired concentration × Final volume) / (Stock concentration) Amount to prepare = (25g × 100mL) / 99g Amount to prepare ≈ 25.25 mL of 99% ethylenediamine 3. Measure and transfer 25.25 mL of the 99% ethylenediamine stock solution into a suitable container or volumetric flask. 4. Calculate the amount of solvent needed: - Since you want to reach a final volume of 100 mL and you already have 25.25 mL of ethylenediamine, you will need to add approximately 74.75 mL of solvent (usually distilled water) to reach the target volume: 100 mL - 25.25 mL = 74.75 mL 5. Using a graduated cylinder, measure and add 74.75 mL of distilled water (or another appropriate solvent) to the container or volumetric flask containing ethylenediamine. 6. Mix the solution thoroughly to ensure that it's homogenous. 7. Label the container with the appropriate information: "25% ethylenediamine in distilled water" and include the volume, storage conditions (e.g., "Store at room temperature"), and any hazard or safety information. Now you have prepared 100 mL of a 25% ethylenediamine solution from a 99% ethylenediamine stock solution. Remember to always use proper personal protective equipment (PPE) and follow safety guidelines when working with chemicals.
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