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Question: For a fictional human tissue, it is hypothesised that cancer stem cells have the following expression pattern of cell surface markers (CD44+/CD24+/ESA+). Flow cytometry was used to isolate this population of cells. explain how to determine what percentage of the total cell population are the putative cancer stem cells. Also, briefly describe two controls that should have been included in the experimental design.
To determine what percentage of the total cell population are the putative cancer stem cells, flow cytometry can be used to sort the cells based on their expression of cell surface markers. Specifically, the cells should be gated based on their expression of CD44, CD24, and ESA, and the percentage of gated cells should be calculated. Two controls that should have been included in the experimental design are a negative control (cells not expressing the markers of interest) and a positive control (cells already known to express the markers). These two controls can be used to validate the flow cytometry results and ensure that the gated cells are, indeed, expressing the target markers.
Jan. 11, 2023, 4:07 a.m.
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