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Question: design an experiment where exosomes change histone modification of stem cell in steps
1. Isolate exosomes from a cell line of interest (e.g. stem cells). 2. In vitro transcribe and translate the exosomes’ RNA into proteins in the presence of a histone modification of interest, such as acetylation or phosphorylation. 3. Combine the exosomes with stem cells in a culture medium. 4. Incubate the cells for 24-48 hours and measure the changes in the histone modification levels through an ELISA assay or western blot. 5. Repeat steps 3 and 4 using different concentrations of the exosomes and/or different histone modifications to assess the effect of each. 6. Compare the results between the different treatments to assess the effect of the exosomes on the stem cells’ histone modification profile.
Jan. 16, 2023, 5:37 a.m.
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