Ask AI: design an experiment of how you will obtain aged exosomes from a mouse in steps

1. Obtain a mouse from a mouse colony and euthanize the mouse via a humane method, such as anesthetic overdose. 2. Collect the mouse's blood via cardiac puncture, and separate the blood from the mouse via centrifuge into serum and red blood cells, taking care to save the serum in a new container. 3. Extract the exosomes from the mouse serum using a commercially available exosome extraction method, such as ultrafiltration. 4. Isolate the exosomes via density gradient centrifugation, and save them in a new container. 5. Place the exosomes in an airtight container and store them in a refrigerator for a period of time, to allow for aging of the exosomes. 6. After the exosomes have been placed in a refrigerator for the desired amount of time, collect the aged exosomes and use them for further study.