Ask AI: Describe step by step what occurs in adenine base editing using ABE8e-NRCH

1. The target adenine base is identified in a single stranded DNA sequence. 2. The ABE8e-NRCH enzyme is then introduced to the target site. This enzyme contains an Adenine deaminase domain that targets the adenine base. 3. The adenine deaminase domain of the enzyme deaminates the target adenine, converting it into inosine (an unstable form of hypoxanthine). 4. The subsequent nucleolytic cleavage of the inosine leaves behind a gap in the DNA sequence. 5. To fill this gap, a new cytosine is then added to the sequence. This restores the original base-pairing and creates a new C:G base-pair. 6. The adenine base has now been successfully edited.